Which temperature is used to differentiate Pseudomonas aeruginosa from Pseudomonas putida?

Which temperature is used to differentiate Pseudomonas aeruginosa from Pseudomonas putida?

• A. 42°C
• B. 37°C
• C. 25°C
• D. 30°C

Answer: (A)

Differentiating by temperature tolerance is a practical way to tell closely related Pseudomonas species apart. Pseudomonas aeruginosa can grow at a higher temperature, specifically 42°C, whereas Pseudomonas putida typically cannot thrive at that elevated temperature. So when you incubate isolates at 42°C, P. aeruginosa is expected to grow while P. putida does not, making this a reliable distinguishing test. At standard body-temperature incubation (37°C), both often grow, so that temperature isn’t helpful for differentiation. Lower temperatures like 25°C or 30°C are less discriminating because many environmental pseudomonads, including some P. putida strains, can grow there as well.

Differentiating by temperature tolerance is a practical way to tell closely related Pseudomonas species apart. Pseudomonas aeruginosa can grow at a higher temperature, specifically 42°C, whereas Pseudomonas putida typically cannot thrive at that elevated temperature. So when you incubate isolates at 42°C, P. aeruginosa is expected to grow while P. putida does not, making this a reliable distinguishing test. At standard body-temperature incubation (37°C), both often grow, so that temperature isn’t helpful for differentiation. Lower temperatures like 25°C or 30°C are less discriminating because many environmental pseudomonads, including some P. putida strains, can grow there as well.