In Rh typing, if AHG is positive at room temperature and at 37C with no check cells performed, the result is best described as what?

In Rh typing, if AHG is positive at room temperature and at 37C with no check cells performed, the result is best described as what?

• A. Rh positive
• B. Rh negative
• C. Invalid Rh typing
• D. Rh indeterminate

Answer: (C)

AHG testing requires a validity check to confirm that a reaction is truly due to the antigen–antibody interaction and not a technical artifact. When the AHG reaction is positive at both room temperature and at 37°C but no check cells are used, there’s no way to verify that the reactivity is specific and reliable. Check cells serve as essential controls to confirm the test is functioning correctly; without them, the result cannot be interpreted as a true Rh type. Therefore, this Rh typing is considered invalid and must be repeated with appropriate controls, including check cells.

AHG testing requires a validity check to confirm that a reaction is truly due to the antigen–antibody interaction and not a technical artifact. When the AHG reaction is positive at both room temperature and at 37°C but no check cells are used, there’s no way to verify that the reactivity is specific and reliable. Check cells serve as essential controls to confirm the test is functioning correctly; without them, the result cannot be interpreted as a true Rh type. Therefore, this Rh typing is considered invalid and must be repeated with appropriate controls, including check cells.